Method of isolating restriction fragment deletions in vibrio...

Details Method of isolating restriction fragment deletions in vibrio... Method of isolating restriction fragment deletions in vibrio...

C - Chemistry, Metallurgy

12

N

167/135, 195/33,

C12N 7/01 (2006.01) A61K 39/106 (2006.01) C07K 14/28 (2006.01) C12N 7/04 (2006.01) C12N 15/01 (2006.01)

Patent

CA 1324968

Abstract This invention relates to a method of isolating deletion mutants of Vibrio cholerae, wherein the deletion is perdetermined by digestion with restriction endonu- cleases of known specificity. The deletions are inserted into the Vibrio cholerae chromosome by in vivo recombina- tion between a plasmid carrying the desired deletion, with adjacent flanking sequences, and the Vibrio cholerae chromosome. The invention includes the isolation and characterization of a new Vibrio cholerae strain having a deletion in the tox gene, as defined by ACC I, Xba I, Cla I and/or restriction endonuclease sites.

448711

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