Method of isolation of rna-binding compounds

Details Method of isolation of rna-binding compounds Method of isolation of rna-binding compounds

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/10 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2318751

The present invention provides a method of isolation of compounds that bind specifically to RNA or that alter the affinity of known compounds for RNA. The method utilises changes in the levels of a fluorescent marker protein expressed in the same cell as a putative RNA-binding compound to detect those compounds of interest.

Procédé permettant d'isoler des composés qui se lient spécifiquement à l'ARN ou qui modifient l'affinité de composés connus pour l'ARN. Ledit procédé repose sur les changements des taux d'une protéine marqueur fluorescente exprimée dans la même cellule qu'un composé susceptible de se lier à l'ARN pour détecter les composés recherchés.

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