Method of killing target cells in harvested cell populations...

A - Human Necessities – 61 – K

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A61K 47/48 (2006.01) A61K 35/14 (2006.01) A61K 35/28 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2248620

A method for killing unwanted target cells in a cell population comprising nucleated cells harvested from peripheral blood, or CD-34+ or similar early progenitor cells selected from the above nucleated cells or from bone marrow aspirates, in which method the cell population is in vitro or in vivo exposed to two or more immunotoxins selectively killing the malignant cells, is described. Furthermore the invention relates to the mixture of immunotoxins, the use of the mixture and a kit for performing the method.

Méthode pour tuer des cellules cibles indésirables dans une population de cellules comprenant des cellules nucléées récoltées dans des cellules génitrices précoces du sang périphérique, de CD-34?+¿ ou similaire choisies parmi lesdites cellules nucléées ou des produits d'aspiration de la moelle épinière, après quoi la population de cellules est exposée in vitro ou in vivo à une ou plusieurs immunotoxines tuant de manière sélective les cellules malignes. L'invention se rapporte, de plus, à un mélange d'immunotoxines, à l'utilisation dudit mélange et à un nécessaire pour la mise en oeuvre de ladite méthode.

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