C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2372162
This invention relates to methods of amplifying nucleic acids to minimize contamination by products of earlier amplification reactions. More particularly, it relates to methods of using nucleic acid labels that inhibit further amplification of the amplicon, and compositions that are useful to accomplish this task. In particular, the present invention relates to photoreactive complexes of a binding ligand, a binding enhancer and a label that exhibit.
La présente invention concerne des méthodes d'amplification d'acide nucléique visant à minimiser la contamination par des produits issus de réactions d'amplification antérieures. L'invention concerne, en particulier, des méthodes d'utilisation de marqueurs d'acide nucléique inhibant toute amplification subséquente de l'amplicon, ainsi que des compositions utilisées à cet effet. L'invention concerne, plus particulièrement, des complexes photoréactifs d'un ligand de liaison, d'un activateur de liaison et d'un marqueur.
Dattagupta Nanibhushan
Sridhar C. Nagaraja
Wu Whei-Kuo
Applied Gene Technologies Inc
Fetherstonhaugh & Co.
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