Method of loading a crystallization device

B - Operations – Transporting – 01 – D

Patent

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Details

B01D 9/00 (2006.01) B01L 3/06 (2006.01) C30B 7/00 (2006.01) C30B 29/58 (2006.01)

Patent

CA 2733381

The present invention pertains to a method for loading a crystallization device and for manufacturing a crystallization device comprising multiple receptacles with a pre-defined amount of at least one matrix-forming compound capable of forming a crystallization matrix for a membrane protein, said method comprising the following steps: a) Modifying the state of aggregation of said at least one matrix-forming compound to a fluidic state which allows dispensing said at least one matrix-forming compound, and b) dispensing a defined amount of said at least one matrix-forming compound into at least one receptacle of the crystallization device, wherein said dispensed matrix-forming compound solidifies within said receptacle. Thereby, prefilled crystallization devices are obtained which can be used as consumables in particular in automated crystallization processes. Also provided are protein crystallization methods using respectively prepared crystallization devices.

La présente invention concerne un procédé de chargement d'un dispositif de cristallisation et de fabrication d'un dispositif de cristallisation qui comprend plusieurs réceptacles qui contiennent une quantité prédéfinie d'au moins un composé de formation de matrice qui peut former une matrice de cristallisation pour une protéine de membrane, ledit procédé comprenant les étapes suivantes : a) faire passer ledit ou lesdits composés de formation de matrice de l'état d'agrégation à un état fluide qui permet de distribuer ledit ou lesdits composés de formation de matrice et b) distribuer une quantité définie dudit ou desdits composés de formation de matrice dans au moins un réceptacle du dispositif de cristallisation, ledit composé de formation de matrice distribué se solidifiant à l'intérieur dudit réceptacle. On obtient ainsi des dispositifs de cristallisation pré-remplis que l'on peut utiliser comme consommables en particulier dans des procédés de cristallisation automatisés. L'invention concerne également des procédés de cristallisation de protéines qui utilisent des dispositifs de cristallisation préparés de manière correspondante.

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