Method of magnetic separation of cells and the like, and...

Details Method of magnetic separation of cells and the like, and... Method of magnetic separation of cells and the like, and...

B - Operations, Transporting

03

C

195/33, 195/44

B03C 1/00 (2006.01) A61K 35/12 (2006.01) C12M 1/00 (2006.01) C12M 3/00 (2006.01) C12N 1/02 (2006.01) C12N 7/02 (2006.01) G01N 33/543 (2006.01) G01N 33/544 (2006.01)

Patent

CA 1127571

METHOD OF MAGNETIC SEPARATION OF CELLS AND THE LIKE, AND MICROSPHERES FOR USE THEREIN ABSTRACT Magnetically-responsive microspheres having Protein A associate with the outer surfaces thereof are reacted with antibodies selective to the cells, bacteria, or viruses to be separated from a mixed population to attach the antibodies in oriented relation with their Fab arms extending outwardly, and the microspheres are then used in a magnetic separation procedure. The preferred microspheres are prepared from a mixture of albumin, Protein A, and magnetic particles, the microspheres being prepared so that the Protein A is present in the exterior surfaces for antibody binding. - 1 -

345986

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