Method of making cs6 antigen vaccine for treating,...

Details Method of making cs6 antigen vaccine for treating,... Method of making cs6 antigen vaccine for treating,...

A - Human Necessities

61

K

A61K 38/17 (2006.01) A61K 39/108 (2006.01) A61P 31/04 (2006.01) C07K 14/245 (2006.01) C12N 15/70 (2006.01) C12P 21/02 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2477273

Disclosed herein are methods for making large amounts of highly pure colonization factors. The methods of the present invention differ from prior art methods in that host cells which express the colonization factor of interest are cultured in media comprising more than about 50 ~g/l of an antibiotic, the media is centrifuged and then filtered with a 0.2 ~m filter tangential flow cartridge and a 300,000 MW cut-off filter, and a divalent cation is added. As disclosed herein the colonization factors made by the method of the present invention may be used in pharmaceutical compositions and methods for treating or preventing enterotoxigenic Escherichia coli infections.

L'invention concerne des méthodes permettant de préparer de grandes quantités de facteurs de colonisation à pureté élevée. Les méthodes de l'invention diffèrent de celles de l'état antérieur de la technique en ce que des cellules hôtes exprimant les facteurs de colonisation d'intérêt sont cultivées dans un milieu comprenant plus d'environ 50 ?g/l d'un antibiotique. Le milieu est centrifugé, puis filtré au moyen d'une cartouche de filtres à écoulement tangentiel de 0.2 ?m et d'un filtre à découpes de 300,000 MW, un cation divalent étant ajouté. Les facteurs de colonisation préparés au moyen de la méthode de l'invention peuvent être utilisés dans des compositions pharmaceutiques et des méthodes permettant de traiter ou de prévenir des infections enterotoxigènes par Escherichia coli.

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