Method of making embryoid bodies from primate embryonic stem...

Details Method of making embryoid bodies from primate embryonic stem... Method of making embryoid bodies from primate embryonic stem...

C - Chemistry, Metallurgy

12

N

C12N 5/0735 (2010.01) C12N 5/073 (2010.01)

Patent

CA 2400158

Primate embryoid bodies are formed from primate ES cells. The ES cells form clumps. One then removes the clumps, as clumps, and permits incubation under non-adherent conditions. The development of embryoid bodies from primate ES cells is dependent on maintaining the aggregation of cells, as individualized cells will rapidly die.

Selon l'invention, des corps embryoïdes de primate sont formés à partir de cellules souches d'embryon de primate. Les cellules souches d'embryon forment des amas. On enlève alors les amas, en tant qu'amas, et on autorise une incubation dans des conditions de non adhérence. Le développement de corps embryoïdes à partir de cellules souches d'embryon de primate dépend du maintien de l'agrégation des cellules, en raison de la mort rapide de cellule individualisée.

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