Method of mapping polypeptide ligand binding regions

Details Method of mapping polypeptide ligand binding regions Method of mapping polypeptide ligand binding regions

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) G01N 33/544 (2006.01) G01N 33/561 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2094798

METHOD OF MAPPING POLYPEPTIDE LIGAND BINDING REGIONS ABSTRACT A method is provided for mapping regions of a polypeptide exhibiting selective affinity for a chemical species. The method comprises the steps of digesting the polypeptide with an exoprotease, substantially free of endoprotease activity, to produce a series of partially digested polypeptides of different molecular weight, but a common undigested terminus. The position of the targeted region in the primary structure of the polypeptide is defined by determining the molecular weight of the lowest molecular weight species exhibiting the selective affinity. The method has particular utility for mapping antibody- binding or receptor-binding epitopes.

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