Method of measurement in biological systems

G - Physics – 01 – N

Patent

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167/40, 150/8, 1

G01N 33/60 (2006.01) A61K 51/04 (2006.01) C12Q 1/68 (2006.01) G01N 23/00 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2027714

Disclosed is a method of quantifying molecules in biological substances, comprising: a. selecting a biological host comprising at least one biological substance in which radioisotopes are present in concentrations equal to or less than the concentration in the ambient biosphere, b. preparing a radioisotope-labeled reactive chemical specie, c. administering the chemical specie to the biological host in doses sufficently low to avoid significant damage to the host's biological system, d. allowing a period of time to elapse sufficient for dissemination and reaction of the chemical specie with the host throughout the biological system of the host, e. isolating a reacted fraction of a sample of the biological substance from the host in a manner sufficient to avoid contamination of the fraction from extraneous sources of the radioisotope, f. converting the fraction of the biological substance to a product material which efficiently produces charged ions in an ion source without introduction of significance isotopic fractionation, and, g. measuring the radioisotope concentration in the product material using an accelerator mass spectrometer.

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