Method of measurement of protease and thin membranes used...

Details Method of measurement of protease and thin membranes used... Method of measurement of protease and thin membranes used...

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01)

Patent

CA 2248272

A method of assaying proteases such as matrix metalloproteases comprising the steps of (1) bringing biological samples such as protease-containing tissue slices or extracts into contact with a thin membrane containing protease substrates and hard coating agents and formed on the surface of a support, and (2) detecting the traces of digestion formed on the membrane by the action of the protease with the naked eye or under a microscope.

Cette invention concerne un procédé d'analyse de protéases, tel que des métalloprotéases matricielles, lequel procédé comprend les étapes suivantes: (1) mettre des échantillons biologiques, comme des tranches ou des extraits de tissus contenant des protéases, en contact avec une membrane fine contenant des substrats de protéase et des agents de revêtement solides formés à la surface d'un support; et (2) déceler à l'oeil nu ou au microscope les traces de digestion qui se sont formées sur la membrane sous l'action de la protéase.

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