Method of measuring the affinity of biomolecules

Details Method of measuring the affinity of biomolecules Method of measuring the affinity of biomolecules

G - Physics

01

N

G01N 33/53 (2006.01) B01J 19/00 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2629931

The invention provides a method of measuring the affinity of first and second biomolecules in which a first biomolecule is tethered by a first tether portion having a first tether portion length and a second biomolecule is tethered by a second tether portion having a second tether portion length, the method comprising determining binding of adjacent first and second biomolecules to each other, varying at least one of the first and second tether lengths and determining binding of the first and second biomolecules. The invention also provides apparatus suitable for use in the method of the invention.

La présente invention concerne un procédé permettant de mesurer l~affinité d~une première et d~une seconde biomolécule. Selon cette affinité, une première biomolécule est attachée par une première partie d~attache possédant une première longueur de partie d~attache et une seconde biomolécule est attachée par une seconde partie d~attache possédant une seconde longueur de partie d~attache. Le procédé consiste à déterminer la liaison des premières et secondes biomolécules adjacentes entre elles, à modifier au moins la première ou la seconde longueur de partie d~attache et à déterminer la liaison de la première et de la seconde biomolécule. L~invention porte également sur un appareil destiné à être utilisé dans le procédé de l~invention.

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