Method of measuring the number of bacteria, device of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/06 (2006.01) C12M 1/34 (2006.01) C12Q 1/10 (2006.01) G01N 27/416 (2006.01)

Patent

CA 2575640

A bacterium counting method comprises steps (a to b). At step (a), a sample (specimen) to be measured and containing a predetermined species of bacterium (for example, escherichia coli or coliform) is added to a predetermined culture medium (for example, a culture medium used for a specific enzyme ground substance culture medium method. At step (b), the value of the current flowing through the culture medium to which the sample is added is measured at a predetermined temperature and at a predetermined constant voltage by using an oxygen electrode. At step (c), the time elapsed from the start of the measurement at step (b) until the current value which has decreased increases and exceeds a predetermined threshold is measured. At step (d), on the basis of the elapsed time, the initial number of bacteria of the species contained in the sample is counted.

Procédé de comptage de bactéries comprenant les étapes (a à b). À l~étape (a), un échantillon (spécimen) à mesurer et contenant une espèce prédéterminée de bactérie (par exemple, escherichia coli ou coliforme) est ajouté à un moyen de culture prédéterminé (par exemple, un moyen de culture utilisé pour un procédé de moyen de culture de substance fondamentale à enzyme spécifique). À l~étape (b), la valeur du courant passant à travers le moyen de culture auquel l~échantillon est ajouté est mesuré à une température prédéterminée et à une tension constante prédéterminée en utilisant une électrode oxygène. À l~étape (c), le temps écoulé depuis le commencement du mesurage à l~étape (b) jusqu~à ce que la valeur de courant qui a décrû augmente et dépasse un seuil prédéterminé est mesuré. À l~étape (d), sur la base du temps écoulé, le nombre initial de bactéries de l~espèce contenue dans l~échantillon est compté.

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