Method of micronization

B - Operations – Transporting – 02 – C

Patent

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B02C 19/06 (2006.01) A61K 9/14 (2006.01)

Patent

CA 2667969

A method for micronization of a dispersion of particles comprising a protein having a predetermined level of biological activity. The method comprises introducing the dispersion into a vortex chamber milling apparatus under milling conditions which result in a protein powder having a particle size distribution of 5 to 100 µm and/or exhibiting a 30 to 400 fold size reduction of the protein particle dispersion from its original size, and retaining at least 80% of the predetermined level of biological activity of the protein. The milling conditions include one or more parameters selected from the following: input pressure between 1 and 7 Bars; injector pressure between 0.2 and 5 Bars; loading rate between 0.1 and 5 kg/hour; and gas flow between 30 and 100 m3/hour.

L'invention concerne un procédé de micronisation d'une dispersion de particules comprenant une protéine dont le niveau d'activité biologique est prédéterminé. Le procédé comporte l'introduction de la dispersion dans un appareil de broyage à chambre de turbulence dans des conditions de broyage qui conduisent à une poudre de protéine dont la distribution granulométrique est de 5 à 100 µm et/ou présentant une réduction de dimension de 30 à 400 fois la dispersion de particules de protéine par rapport à sa dimension initiale, et conservant au moins 80% du niveau prédéterminé de l'activité biologique de la protéine. Les conditions de broyage comprennent un ou plusieurs paramètres choisis parmi les suivants : pression d'entrée entre 1 et 7 bars, pression d'injecteur entre 0,2 et 5 bars, vitesse de charge entre 0,1 et 5 kg/heure; et écoulement du gaz entre 30 et 100 m3/heure.

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