Method of modifying genome in higher plant

Details Method of modifying genome in higher plant Method of modifying genome in higher plant

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) C12N 5/14 (2006.01) C12N 15/84 (2006.01)

Patent

CA 2460617

There has been established a technique of modifying a part of a genome sequence with the use of homologous recombination wherein it has been considered difficult to achieve a high efficiency and a high reproducibility in higher plants. Thus, it becomes possible to obtain an individual undergoing homologous recombination at a high efficiency and a high reproducibility targeting the inherent genome sequence of a higher plant without altering the original locus. Moreover, it is possible to construct a transformant plant having an arbitrarily altered specific gene, thereby contributing to the analysis of gene functions and the clarification of gene expression mechanism associating changes in genomic dynamics.

Selon une technique mise au point pour modifier une partie d'une séquence génomique par recombinaison homologue, on a constaté qu'il était difficile d'obtenir un degré élevé d'efficacité et de reproductibilité chez les plantes supérieures. Selon l'invention, il est possible d'obtenir un individu soumis à une recombinaison homologue avec un degré élevé d'efficacité et de reproductibilité en ciblant la séquence génomique inhérente d'une plante supérieure sans modifier le locus d'origine. Par ailleurs, il est possible de construire une plante transformée possédant un gène spécifique modifié arbitrairement, ce qui contribue à l'analyse des fonctions géniques et à la clarification des changements associés au mécanisme de l'expression génique en dynamique génomique.

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