G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/52 (2006.01) C12Q 1/56 (2006.01) G01N 21/25 (2006.01) G01N 21/78 (2006.01) G01N 33/86 (2006.01) G01N 21/31 (2006.01)
Patent
CA 2069887
A method for measuring the concentration of a reagent in a reaction mixture including the steps of: adding dye to a reagent until the dye is at a given concentration in the reagent; mixing the reagent with a specimen to form a reaction mixture, wherein the specimen comprises a component which reacts with the reagent to form a reaction product; measuring the formation of reaction product at a first spectral region; measuring the concentration of dye in the reaction mixture at a second spectral region in which the dye has an optical characteristic, such as absorption or fluorescence, the second spectral region being different from the first spectral region; and determining the concentration of the reagent in tie reaction mixture based on the concentration of dye measure. In a further aspect of the invention there is provided a reagent containing a dye useful in the above method.
Driscoll Richard Cornelius
Fischer Timothy J.
Akzo N.v.
Biomerieux Inc.
Na
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