Method of nuclear reprogramming

Details Method of nuclear reprogramming Method of nuclear reprogramming

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2695522

This invention provides a method of producing an induced pluripotent stem cell comprising the step of introducing at least one kind of non-viral expression vector (more preferably a plasmid vector) incorporating at least one gene that encodes a reprogramming factor into a somatic cell. An induced pluripotent stem cell wherein no exogenous genes induced is integrated into the cellular genome is also provided.

La présente invention concerne un procédé de production dune cellule souche pluripotente induite comprenant létape consistant à introduire au moins un type de vecteur dexpression viral (plus préférablement un vecteur plasmidique) comprenant au moins un gène qui code pour un facteur de reprogrammation dans une cellule somatique. La présente invention concerne en outre une cellule souche pluripotente induite dans laquelle aucun gène exogène induit nest intégré dans le génome cellulaire.

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