Method of nucleic acid detection using target-specific assays

Details Method of nucleic acid detection using target-specific assays Method of nucleic acid detection using target-specific assays

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12M 1/36 (2006.01) G01N 1/30 (2006.01) G01N 31/22 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2394974

Homogeneous and multiplex assays for target nucleic acids hybridized to oligonucleotide probes immobilized on particulate and non-particulate substrates are described. Target nucleic acids are determined using detection methods, such as flow cytometric methods, capable of particle discrimination based on the light scattering or fluorescence properties of the particle, or by spatial resolution of oligonucleotides immobilized at specific loci on a substrate. Target-correlated fluorescence signal, originating from a target nucleic acid hybridized to the substrate-immobilized oligonucleotide is determined as a measure of the presence or amount of the target nucleic acid.

L'invention concerne des dosages homogènes et multiplex d'acides nucléiques cibles hybridés à des sondes oligonucléotidiques immobilisées sur des substrats particulaires et non particulaires. Les acides nucléiques cibles sont déterminés à l'aide de méthodes de détection, telles que des méthodes de cytométrie de flux, capables d'une discrimination des particules sur la base de la diffusion de la lumière ou de propriétés de fluorescence de la particule, ou par une résolution spatiale d'oligonucléotides immobilisés au niveau de loci spécifiques sur un substrat. Un signal de fluorescence en corrélation avec la cible, provenant d'un acide nucléique cible hybridé à l'oligonucléotide immobilisé sur substrat, est déterminé en tant que mesure de la présence ou de la quantité de l'acide nucléique cible.

Affiliated with

Also associated with

Rating

Method of nucleic acid detection using target-specific assays does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of nucleic acid detection using target-specific assays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of nucleic acid detection using target-specific assays will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-2073555