Method of nucleic acid sequence selection

Details Method of nucleic acid sequence selection Method of nucleic acid sequence selection

C - Chemistry, Metallurgy

12

N

C12N 15/00 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2169283

The present invention provides a method for the rapid isolation and recovery of a desired target DNA or RNA molecules from a mixture or library containing such molecules. The method involves the use of biotinylated probes and enzymatic repair-cleavage to eliminate undesired library members from a sample.

L'invention porte sur un procédé rapide d'isolement et de recueil de molécules d'un ADN ou d'un ARN cible donné à partir d'un mélange ou d'une banque les contenant. Ledit procédé consiste à utiliser des sondes biotinylées et des réparations/clivages enzymatiques pour éliminer les éléments de la banque indésirables contenus dans l'échantillon.

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