Method of obtaining a composition containing...

C - Chemistry – Metallurgy – 07 – C

Patent

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C07C 403/24 (2006.01) C12S 99/00 (2010.01) C07C 29/74 (2006.01) C07C 403/00 (2006.01) C12N 1/00 (2006.01) C12N 1/12 (2006.01) C12P 23/00 (2006.01)

Patent

CA 2234332

There are disclosed a composition containing 9-cis .beta.- carotene in high-purity and an industrially applicable method of obtaining the same from an alga. The composition can be obtained through steps of washing with ethanol dry powder of the alga belonging to Dunaliella, adding hexane to the washed algal powder, stirring the same, subjecting the same to filtration to obtain a filtrate, concentrating the filtrate to obtain a semi-solidic concentrate, adding hexane to the semi-solidic concentrate in an amount of 10 - 18ml of hexane to 1g of the semisolidic concentrate, stirring the same, subjecting the same to filtration to obtain a filtrate, concentrating the filtrate to obtain an oily concentrate, adding hexane to the oily concentrate in an amount of 2 - 4.5ml of hexane to 1g of the oily concentrate to dissolve the same, and leaving the solution, as it is, under lower temperature condition to cause a separation of the composition. The composition is a rubiginous powder or crystals and contains 9-cis .beta.-carotene in an amount of 75% or more to total amount of the composition.

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