Method of obtaining a retrovirus-containing fraction from...

C - Chemistry – Metallurgy – 12 – N

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C12N 7/00 (2006.01) C12N 7/02 (2006.01) C12Q 1/04 (2006.01) C12Q 1/48 (2006.01) C12Q 1/70 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01)

Patent

CA 1315726

ABSTRACT The invention relates to a method of obtaining a retrovirus-containing fraction, and in particular, though not exclusively, to obtaining a retrovirus containing fraction from mononuclear cells containing a retrovirus. The method of forming a retrovirus-containing fraction from mononuclear cells containing said virus includes preparing a suspension of separated mononuclear cells in a culture medium, incubating said culture and separating the culture supernatant from said incubated culture, and is characterized in that an effective amount of a glucocorticoid or of a leukemia virus or other viral inducing drug is added to the suspension before and/or during the incubation of said cultures. If desired, a retrovirus containing fraction is separated from the separated supernatant.

558426

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