Method of passively adsorbing immuno-reactive haptens to...

Details Method of passively adsorbing immuno-reactive haptens to... Method of passively adsorbing immuno-reactive haptens to...

G - Physics

01

N

167/44

G01N 33/78 (2006.01) G01N 33/543 (2006.01) G01N 33/545 (2006.01)

Patent

CA 1146853

39/014) ABSTRACT OF THE DISCLOSURE The method comprises covalently binding an immuno-reactive to a selected macromolecular carrier and then contacting the resulting hapten-carrier conjugate at a selected concentration in a liquid phase with a selected solid phase until a desired amount of the hapten-carrier conjugate is adsorbed to the surface of the solid phase. Unbound hapten-carrier conjugate is then separated from the solid phase, and the solid phase containing the bound hapten-carrier conjugate is recovered for use in quantitative immunoassays and the like. The solid phase can be, for example, surfaces of a test tube or microtiter well or the like. The method is simple and inexpensive and permits hapten assays of improved sensitivity.

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