G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 21/63 (2006.01) C12Q 1/26 (2006.01) C12Q 1/32 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01) G01N 21/03 (2006.01) G01N 21/25 (2006.01) G01N 21/64 (2006.01) G01N 33/551 (2006.01) G01N 33/552 (2006.01) G01N 33/553 (2006.01) G02B 5/20 (2006.01)
Patent
CA 2446236
The invention relates to a method of performing a fluorescence assay. According to the invention a filter is integrated with at least one component chosen from i) a body comprising a well, wherein the well in which the assay is being performed is exposed to excitation light in such a manner that the filter is positioned between the light-sensitive element and the excitation light source, and ii) a light-sensitive element, wherein the filter is applied at least at the light-sensitive side of the surface of the light-sensitive element, and any light that may be emitted is detected by means of the light- sensitive element. The invention also relates to an apparatus suitable for carrying out the method, as well as a method for its manufacture.
L'invention concerne un procédé destiné à réaliser une analyse par fluorescence. Selon l'invention, un filtre est intégré dans au moins un composant choisi parmi (i) un corps comprenant une cupule dans laquelle l'analyse est réalisée, cette cupule étant exposée à une lumière d'excitation de façon que le filtre soit situé entre l'élément photosensible et la source de la lumière d'excitation, et (ii) un élément photosensible. Le filtre est mis en oeuvre au moins sur le côté photosensible de la surface de l'élément photosensible, une lumière susceptible d'être émise étant détectée au moyen dudit élément photosensible. L'invention concerne également un appareil permettant la mise en application de ce procédé, ainsi que son procédé de fabrication.
Iordanov Ventzeslav Petrov
Sarro Pasqualina Maria
Vellekoop Michael Johannes
Wolffenbuttel Reinoud Felix
Moffat & Co.
Technische Universiteit Delft
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