G - Physics – 01 – N
Patent
G - Physics
01
N
167/37, 73/55
G01N 33/563 (2006.01) G01N 33/544 (2006.01) G01N 33/564 (2006.01)
Patent
CA 1225586
Inventor: A. Nuri Ozkan Title: METHOD OF PERFORMING AN IMMUNO ASSAY, ARTICLE FOR USE THEREIN AND METHOD FOR MAKING SUCH ARTICLE ABSTRACT OF THE DISCLOSURE An immuno-assay of a specimen of a serum or the like to determine immune complexes, includes producing on a plastic base a layer of a non-proteinaceous, non-ionic polymer which will ad- here to the plastic base and has the capability of absorbing immune complexes of the specimen, placing a specimen on the layer and treating the layer to produce an indication of the amount of immune complexes. The polymer may be polyethylene glycol, dex- tran, polyvinyl chloride, a polymeric polyol or an adduct of polyethylene glycol. Washing with conventional solutions, addition of an anti-human IgG couple with an enzyme and addition of a sub- strate reactive therewith, are similar to the ELISA test, with color measurement as by spectrophotometer. Or, the addition of anti IgG-I125 and measurement by a scintillation counter may be used. The ethylene glycol may range in molecular weight from 2,000 to 20,000, although 6,000 to 8,000 is preferred. A product for use in such an assay is a plate having wells or a test tube formed of plastic, polystyrene and polyvinyl chloride being pre- ferred, with a layer of such non-proteinaceous, non-ionic layer on the plate wells or the cavity of the test tube. This product, if it is not to be used immediately, should be provided with a layer or film protecting the polymer layer from the air. ?
424919
Biostar Medical Products Inc.
Fetherstonhaugh & Co.
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