Method of plasmid dna production and purification

C - Chemistry – Metallurgy – 12 – N

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12N 15/10 (2006.01) C07H 1/08 (2006.01) C12N 1/06 (2006.01) C12N 15/70 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2244115

A scalable method for the production of highly purified plasmid DNA in Escherichia coli is described, which method includes growing plasmid-containing cells to a high biomass in exponential growth and lysing the cells by raising the pH of the culture to a carefully controlled pH value in which chromosomal DNA is denatured but plasmid DNA is reversibly renatured. The method has been developed for the production of pharmaceutical grade DNA for use in in vivo and ex vivo gene therapy.

L'invention concerne un procédé à architecture évolutive pour la production d'ADN plasmide fortement purifié dans l'Escherichia Coli. Ce procédé consiste à cultiver des cellules contenant du plasmide jusqu'à une biomasse élevée, en croissance exponentielle, et à lyser ces cellules en augmentant le pH de la culture jusqu'à une valeur de pH contrôlée avec soin, à laquelle l'ADN chromosomique est dénaturé, mais l'ADN plasmide est renaturé de manière réversible. Le procédé a été mis au point pour la production d'ADN de qualité pharmaceutique, prévu pour être utilisé in vivo et ex vivo en thérapie génique.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Method of plasmid dna production and purification does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of plasmid dna production and purification, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of plasmid dna production and purification will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1614938

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.