C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C07H 1/08 (2006.01) C12N 1/06 (2006.01) C12N 15/70 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2244115
A scalable method for the production of highly purified plasmid DNA in Escherichia coli is described, which method includes growing plasmid-containing cells to a high biomass in exponential growth and lysing the cells by raising the pH of the culture to a carefully controlled pH value in which chromosomal DNA is denatured but plasmid DNA is reversibly renatured. The method has been developed for the production of pharmaceutical grade DNA for use in in vivo and ex vivo gene therapy.
L'invention concerne un procédé à architecture évolutive pour la production d'ADN plasmide fortement purifié dans l'Escherichia Coli. Ce procédé consiste à cultiver des cellules contenant du plasmide jusqu'à une biomasse élevée, en croissance exponentielle, et à lyser ces cellules en augmentant le pH de la culture jusqu'à une valeur de pH contrôlée avec soin, à laquelle l'ADN chromosomique est dénaturé, mais l'ADN plasmide est renaturé de manière réversible. Le procédé a été mis au point pour la production d'ADN de qualité pharmaceutique, prévu pour être utilisé in vivo et ex vivo en thérapie génique.
Hanak Julian Alexis
Hitchcock Antony Gordon
Thatcher David Robert
Varley Diane Lesley
Cobra Biologics Limited
Cobra Therapeutics Limited
Kirby Eades Gale Baker
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