Method of plastid transformation in asteraceae, vector for...

Details Method of plastid transformation in asteraceae, vector for... Method of plastid transformation in asteraceae, vector for...

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) C07K 14/11 (2006.01)

Patent

CA 2494986

The present invention relates to a method for the transformation of plastid genomes of plant species, in particular Asteraceae plant species, comprising the steps of providing a transformation vector carrying a DNA sequence of interest; subjecting a plant material, which comprises plastids, to a transformation treatment in order to allow the plastids to receive the transformation vector; placing the thus treated plant material for a period of time into contact with a culture medium without selection agent; subsequently placing the plant material into contact with a culture medium comprising a selection agent; and refreshing the culture medium comprising a selection agent to allow plant material comprising plastids that have acquired the DNA of interest to grow into transformants.

L'invention concerne un procédé de transformation de génomes de plastes d'une espèce végétale, en particulier l'espèce Asteraceae. Ce procédé comprend les étapes qui consistent: à utiliser un vecteur de transformation comportant une séquence ADN d'intérêt; à soumettre un matériel végétal, qui comprend des plastes, à un traitement de transformation pour permettre aux plastes de recevoir ledit vecteur de transformation; à mettre le matériel végétal ainsi traité en contact, pendant une certaine période, avec un milieu de culture ne comprenant pas d'agent de sélection; à mettre ensuite ledit matériel végétal en contact avec un milieu de culture comprenant un agent de sélection; et à rafraîchir ledit milieu de culture comprenant un agent de sélection pour permettre aux végétaux comprenant des plastes qui ont acquis l'ADN d'intérêt de croître sous forme de transformant.

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