Method of predicting drug-induced phospholipidosis

Details Method of predicting drug-induced phospholipidosis Method of predicting drug-induced phospholipidosis

C - Chemistry, Metallurgy

12

Q

C12Q 1/34 (2006.01) G01N 33/15 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2697988

The present invention provides a method of predicting drug-induced phospholipidosis, comprising a step of contacting a mammalian cell with a test compound, a step of measuring extracellular and/or intracellular lysosomal enzyme level or activity, or measuring intracellular LC3 level, and a step of selecting a test compound that has enhanced extracellular secretion of the enzyme or increased the protein level as a compound capable of inducing drug-induced phospholipidosis.

La présente invention concerne un procédé de prédiction d'une phospholipidose induite par les médicaments, comprenant une étape de mise en contact de cellules de mammifère avec un composé test ; une étape de mesure de l'activité d'une enzyme lysosomiale à l'extérieur et/ou à l'intérieur des cellules, ou de mesure de la quantité de LC3 présente dans les cellules ; et une étape de sélection d'un composé test favorisant la sécrétion de l'enzyme hors des cellules ou entraînant une augmentation de la quantité de protéine, en tant que composé capable d'induire une phospholipidose induite par les médicaments.

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