Method of preferential labelling of a phycobiliprotein with...

Details Method of preferential labelling of a phycobiliprotein with... Method of preferential labelling of a phycobiliprotein with...

C - Chemistry, Metallurgy

07

K

C07K 14/405 (2006.01) G01N 33/533 (2006.01)

Patent

CA 2114837

2114837 9303062 PCTABS00019 A method for preparing a phycobiliprotein-Texas Red conjugate which overcomes the energy transfer/fluorescent quenching dilemma is disclosed. A phycobiliprotein, such as phycoerythrin, is conjugated with dye, such as Texas Red, in the presence of a selective salt which causes a hydrophobic intramolecular rearrangement of the phycobiliprotein thereby exposing more hydrophobic sites for binding to Texas Red. The conjugate is useful in multiple color fluorescence assays without requiring the use of multiple exciting sources.

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