Method of preferential labelling of phycobiliprotein with...

Details Method of preferential labelling of phycobiliprotein with... Method of preferential labelling of phycobiliprotein with...

G - Physics

01

N

G01N 33/533 (2006.01) C07K 1/13 (2006.01) G01N 33/542 (2006.01) G01N 33/72 (2006.01)

Patent

CA 2143757

A method for preparing phycobiliprotein/amine- reactive dye conjugates is disclosed in which the conjugates so prepared overcome the energy transfer/fluorescent quenching dilemma encountered in the use of prior art conjugates. A phycobiliprotein, for example, phycoerythrin or allophycocyanin, is conjugated with an amine-reactive dye, for example, Texas Red or carboxyfluorescein succinimidyl ester, in the presence of a selective salt which causes a hydrophobic intramolecular rearrangement of the phycobiliprotein thereby exposing more hydrophobic sites for binding to the amine-reactive dye. The conjugates prepared according to the invention are useful in multiple color fluorescence assays without requiring the use of multiple exciting sources.

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