B - Operations – Transporting – 01 – D
Patent
B - Operations, Transporting
01
D
204/99.04
B01D 57/02 (2006.01) G01N 27/26 (2006.01) G01N 27/447 (2006.01)
Patent
CA 1152023
METHOD OF PREPARATIVE, KOHLRAUSCH-REGULATED ELECTROPHORESIS USING POLYETHYLENE GLYCOL DERIVATIZED TRAILING IONS Abstract of the Disclosure An improved method of Kohlrausch-regu- lated electrophoresis (isotachophoresis) is dis- closed for the preparative separation of respec- tive macromolecular components such as proteins in a crude sample. The preferred method involves use of a substantially linear, polyethylene glycol derivatized trailing ion having a relatively high mass to charge ratio and a low mobility which is substantially independent of pH changes over a relatively broad pH range. The most preferred trailing ion is a polyethylene glycol dicarboxylic acid having a molecular weight of about 6,000 - 7,500, a pKa value of about 3.7, and a fully ionized mobility of about -1.3 x 10-5 cm.2/volt/ sec. The method hereof permits large scale pro- tein separations and subsequent collection of purified protein fractions over a wide range of pH conditions and without interfering reactions between the proteins and trailing ion. (Docket No. 17091)
373756
Cunningham Bryce A.
Gowling Lafleur Henderson Llp
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