Method of preparing a chromatography matrix

Details Method of preparing a chromatography matrix Method of preparing a chromatography matrix

B - Operations, Transporting

01

J

B01J 20/30 (2006.01) B01J 20/24 (2006.01) B01J 20/291 (2006.01)

Patent

CA 2577822

The present invention relates to a method of preparing a cross-linked polysaccharide chromatography matrix, which comprises to provide an aqueous solution of a gellable polysaccharide, wherein part of the hydroxyl groups are substituted with groups which are not susceptible to nucleophilic attack; to provide essentially spherical droplets of the substituted polysaccharide solution; to form a gel of the substituted polysaccharide solution; and to cross-link the gel. The invention also encompasses a chromatography column packed with a matrix so prepared as well as use thereof in e.g. protein purification.

Procédé de préparation d'une matrice de chromatographie à base de polysaccharide réticulé, qui consiste à préparer une solution aqueuse d'un polysaccharide pouvant être gélifié dans laquelle une partie des groupes hydroxyle sont substitués par des groupes qui ne sont pas sensibles à l'attaque nucléophile, à former des gouttelettes essentiellement sphériques de la solution de polysaccharide substitué, à former un gel à partir de la solution de polysaccharide substitué et à réticuler le gel. La présente invention concerne également une colonne de chromatographie remplie d'une matrice ainsi préparée, ainsi que l'utilisation de ladite colonne pour la purification de protéines par exemple.

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