Method of preparing a viral vector by homologous...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/86 (2006.01) A61K 31/70 (2006.01) A61K 48/00 (2006.01) C12N 7/00 (2006.01) C12N 7/01 (2006.01) C12N 15/10 (2006.01) C12N 15/12 (2006.01) C12N 15/861 (2006.01)

Patent

CA 2182303

Method of preparing within a procaryotic cell a recombinant viral vector by intermolecular homologous recombination, method of preparing an infectious viral particle containing such a vector and pharmaceutical composition comprising said vector or particle. The invention also concerns the therapeutic use of said vector or particle, especially in human gene therapy.

L'invention concerne un procedé pour préparer dans une cellule procaryote un vecteur viral recombinant par recombinaison homologue intermoléculaire, un procédé pour préparer une particule virale infectieuse contenant un tel vecteur, ainsi qu'une composition pharmaceutique les comprenant. L'invention couvre également leur usage thérapeutique, notamment dans le cadre de la thérapie génique appliquée à l'homme.

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