Method of preparing cross-linked enzyme particles

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 9/96 (2006.01) C12N 9/04 (2006.01) C12N 9/78 (2006.01) C12N 11/02 (2006.01) C12N 11/10 (2006.01) C12P 7/00 (2006.01)

Patent

CA 2505402

The invention relates to a method of preparing cross-linked enzyme particles using a cross-linking agent. According to the invention, the enzyme particles are formed and subsequently cross-linked using a cross-linking agent having at least n reactive groups where N >= 3 and a molecular weight of > 2,000 Da. The method according to the invention allows for obtaining enzyme particles having a higher enzyme activity than enzyme particles cross-linked according to the state of the art.

La présente invention concerne une technique de préparation de particules d'enzymes réticulées au moyen d'un agent de réticulation. Selon cette invention, les particules d'enzymes sont formées et par la suite réticulées au moyen d'un agent de réticulation possédant au moins n groupes réactifs avec n= 3 et un poids moléculaire > 2,000 Da. La technique de cette invention permet d'obtenir des particules d'enzymes possédant une plus grande activité enzymatique que les particules d'enzymes réticulées connues jusqu'à présent.

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