Method of preparing eggs for nuclear transfer and uses thereof

Details Method of preparing eggs for nuclear transfer and uses thereof Method of preparing eggs for nuclear transfer and uses thereof

A - Human Necessities

01

K

A01K 67/027 (2006.01) C12N 15/87 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2469257

A method of enucleating an egg by exposing the egg to gamma irradiation. The dose of gamma irradiation used is sufficiently high to halt development of the egg or embryo derived therefrom directed by the nucleus of the irradiated egg, but sufficiently low to enable development of the egg or embryo directed by a nucleus transferred into the enucleated egg.

La présente invention concerne une méthode d'énucléation d'un ovocyte qui consiste à exposer l'ovocyte à un rayonnement gamma. La dose de rayonnement gamma est suffisamment importante pour arrêter la croissance de l'ovocyte ou de l'embryon provenant de ce dernier, sous le contrôle du noyau de l'ovocyte irradié, mais suffisamment faible pour permettre la croissance de l'ovocyte ou de l'embryon, sous le contrôle d'un noyau transféré dans l'ovocyte énucléé.

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