Method of preparing gene expression profile

Details Method of preparing gene expression profile Method of preparing gene expression profile

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12M 1/00 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2500209

The present invention provides a method for preparing a gene expression profile with a high precision, by carrying out the annealing of primers to templates in the temperature range of TmMAX + 6°C to TmMAX + 14°C of the primer in a PCR step of a HiCEP method.

Pour mettre au point une méthode permettant de préparer un profil d'expression génétique à haute précision, une amorce matrice est recuite à une température de matrice comprise entre TmMAX+6 ·C et TmMAX+14 ·C dans l'étape PCR d'un procédé HiCEP.

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