C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.12, 150/8.
C12N 15/00 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2005927
ABSTRACT The method comprises providing a first target recognition moiety having a first specific end sequence of at least about 4 nucleotide bases; providing a second signal-generating moiety having multiple label attached thereto and having a second specific end sequence of at least about 4 nucleotide bases, providing a third bridging complement comprising a nucleotide sequence of about 8-25 nucleotides, at least about 4 of the nucleotides in the bridging complement being capable of hybridizing to the first specific end sequence of the target recognition moiety and at least about 4 other nucleotides in the bridging complement capable of hybridizing to the second specific end sequence of the signal-generating moiety; allowing, under appropriate hybridization conditions, hybridization of the bridging complement to at least a part of the first specific end sequence of the target recognition moiety and at least a part of the second specific end sequence of the signal generating moiety to form a hybridized complex of all three. The 3' terminus of one of the moieties is aligned with the 5' terminus of the other, the 3' terminus and the 5' end being positioned relative to one another in such a manner as to allow formation of a 3'-5' sugar-phosphate link between the first and second moieties. The last step includes contacting the complex with a DNA ligating device in such an amount and for such a period of time as is effective to allow formation of the 3'-5' sugar-phosphate link between the 3' terminus and the 5' terminus of the first and second moieties, to produce a nucleotide probe comprising the first target recognition moiety and the second signal generating moiety covalently attached to one another. This method provides an interesting alternative to the synthesis of nucleic acid probes known to the art.
Bahl Chander
Mcmahon Frank J.
Mendoza Leopoldo
Ortho Diagnostic Systems Inc.
Swabey Ogilvy Renault
LandOfFree
Method of preparing nucleotide probes using a bridging... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of preparing nucleotide probes using a bridging..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of preparing nucleotide probes using a bridging... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1658582