Method of preparing starch hydrolysates by enzymatic hydrolysis

C - Chemistry – Metallurgy – 12 – P

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195/138, 195/68

C12P 19/00 (2006.01) C13K 1/06 (2006.01)

Patent

CA 1039671

A B S T R A C T The invention concerns the preparation of starch hydrolysates by enzymatic liquefaction followed by enzymatic saccharification, said prepara- tion comprising the steps of (1) subjecting a starch slurry containing a liquefaction enzyme to an initial liquefaction at a temperature of between 70 and 90°C, (2) heating the initially liquefied starch medium to a tempera- ture of between 100 and 140-C, (3) cooling the medium to a temperature of between 70 and 90°C and adding a further amount of liquefaction enzyme tofurther liquefy the starch medium, (4) heating the medium to a temperature of above 100°C, (5) cooling the medium to a temperature of between 70 and 90°C and adding a further amount of liquefaction enzyme thereto to complete the liquefaction and (6) cooling the liquefied medium to a temperature of between 50 and 65°C and adding a saccharification. enzyme in order to complete the hydrolysis. The thus prepared starch hydrolysates have high dextrose equival- ent (D.E.) values and satisfactory filtration properties, said preparation being suitable for the conversion of potato, moniac, sorghum and sago starches but particularly useful for wheat and rice starch conversion.

223135

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