Method of preparing transgenic organism with use of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/09 (2006.01) A01K 67/027 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2531367

A technique for efficiently introducing a foreign gene in cells with the use of transposon. In particular, a technique for efficiently preparing a transgenic organism with the use of transposon having its transposition activity strikingly enhanced through methylation of a sequence containing transposon. The methylation is retained even after incorporation in a genome, and now can be utilized in actual gene incorporation in a genome. This technique can realize strikingly efficient gene transformation as compared with the conventional method of preparing a transgenic organism with the use of transposon.

Technique servant à introduire de façon efficace un gène étranger dans des cellules à l'aide d'un transposon. En particulier, technique servant à préparer de façon efficace un organisme transgénique à l'aide d'un transposon dont l'activité de transposition est considérablement amplifiée par l'intermédiaire de la méthylation d'une séquence contenant ce transposon. La méthylation perdure même après incorporation dans un génome et peut être utilisée maintenant dans l'incorporation réelle d'un gène dans un génome. Cette technique permet de réaliser une transformation génique extrêmement efficace par rapport au procédé classique de préparation d'un organisme transgénique au moyen d'un transposon.

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