Method of preventing virus: cell fusion by inhibiting the...

C - Chemistry – Metallurgy – 07 – K

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C07K 14/11 (2006.01) A61K 38/16 (2006.01) A61K 39/42 (2006.01) A61P 31/16 (2006.01) C07K 14/005 (2006.01) C07K 16/10 (2006.01) C12N 15/44 (2006.01)

Patent

CA 2544848

The present invention relates to a method of preventing or inhibiting viral infection of a cell and/or fusion between the envelope of a virus and the membranes of a cell targeted by the virus (thereby preventing delivery of the viral genome into the cell cytoplasm, a step required for. viral infection). The present invention particularly relates to the families of RNA viruses, including the arenaviruses, coronaviruses, filoviruses, orthomyxoviruses, paramyxoviruses, and retroviruses, having Class I membrane fusion proteins as the fusion proteins that mediate this fusion process. The present invention provides for a method of identifying a conserved motif or domain called the fusion initiation region (FIR) in these viruses. The present invention further provides for methods of preventing infection by such viruses, by interfering with their FIR. The present invention further provides for methods of treatment and prophylaxis of diseases induced by such viruses.

La présente invention concerne une méthode permettant d'empêcher ou d'inhiber l'infection virale d'une cellule et/ou la fusion entre l'enveloppe d'un virus et les membranes de la cellule visée par le virus (ce qui empêche le passage du génome viral dans le cytoplasme de la cellule, opération nécessaire pour l'infection virale). L'invention concerne en particulier les familles de virus à ARN, dont les arénavirus, coronavirus, filovirus, orthomyxovirus, paramixovirus et rétrovirus, possédant des protéines de fusion à membrane de classe I comme protéines de fusion induisant le processus de fusion. Avec cette invention, on dispose d'une méthodepermettant d'identifier un motif ou un domaine conservés dit région d'initiation de fusion (FIR) dans ces virus. Cette invention porte en outre sur des méthodes propres à empêcher une infection par lesdits virus en interférant avec leur FIR. Sont également décrites des méthodes de traitement et de prophylaxie de maladies induites par de tels virus.

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