Method of processing and preserving collagen based tissues

A - Human Necessities – 01 – N

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A01N 1/00 (2006.01) A01N 1/02 (2006.01)

Patent

CA 2320887

A process for the preserving collagen-based tissues involves procuring the collagen-based tissue; treating the tissue in a detergent solution; treating the tissue in an enzyme solution; treating the tissue so as to prevent or inhibit the molecular crosslinking of processed tissues via the Maillard reaction and the subsequent formation of advanced glycosylation end products; treating the tissue so as to prevent or inhibit the molecular crosslinking of processed tissues via reactive oxidative species of molecules; treating the tissue so as to prevent or inhibit the molecular crosslinking of processed tissues via the formation and propagation of molecular free radicals; treating the tissue in a cryopreservation solution; and cryopreserving the tissue. The process may be utlized to preserve several differing types of collagen based tissue including heart valve, vascular grafts including veins and arteries, umbilical vessels, nerve and nervous system tissue, dura, dermis and other similar collagen based tissues.

L'invention concerne un procédé permettant de conserver des tissus à base de collagène, consistant à obtenir le tissu à base de collagène; à traiter le tissu dans une solution détergente; à traiter le tissu dans une solution d'enzymes; à traiter le tissu de manière à empêcher ou à inhiber la réticulation moléculaire des tissus traités via la réaction de Maillard et la formation ultérieure de produits finaux de glycosylation avancée; à traiter le tissu de manière à empêcher ou à inhiber la réticulation moléculaire des tissus traités via des espèces moléculaires oxydatives réactives; à traiter le tissu de manière à empêcher ou à inhiber la réticulation moléculaire des tissus traités via la formation et la propagation de radicaux libres moléculaires; à traiter le tissu dans une solution de cryopréservation; et à réaliser la cryopréservation du tissu. Ce procédé peut être utilisé pour conserver différents types de tissu à base de collagène à savoir la valvule cardiaque, les greffons vasculaires notamment les veines et les artères, les vaisseaux ombilicaux, les nerfs, les tissus nerveux, la dure-mère, le derme et autres tissus semblables à base de collagène.

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