Method of producing a chimeric protein

Details Method of producing a chimeric protein Method of producing a chimeric protein

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/08 (2006.01) C07K 14/435 (2006.01) C07K 19/00 (2006.01) C12N 7/00 (2006.01) C12N 7/01 (2006.01) C12N 15/40 (2006.01) C12N 15/57 (2006.01) C12N 15/82 (2006.01) C12N 15/83 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2202761

A method of producing a chimeric protein from i.e. a plant virus coding for such a protein. The method allows the production of large (i.e. 25 kDa) proteins which assemble with the virus in infected host cells and are arranged on the outer surface of chimeric viruses. A vector for the production of biologically useful proteins in such a manner is also disclosed.

Un procédé permet de produire une protéine chimère par exemple à partir d'un virus de plantes qui code pour une telle protéine. Ce procédé permet de produire des protéines de grandes dimensions (par exemple 25 kDa) qui se joignent aux virus dans des cellules hôtes infectées et qui se positionnent sur la surface extérieure des virus chimères. L'invention concerne également un vecteur utile pour produire de cette façon des protéines biologiquement utiles.

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