G - Physics – 01 – N
Patent
G - Physics
01
N
167/37
G01N 33/53 (2006.01) B01J 13/00 (2006.01) B01J 13/10 (2006.01) B01J 13/22 (2006.01) G01N 33/532 (2006.01) G01N 33/553 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2026409
A method to produce a reagent containing a narrow distribution of metal sol particles of a selected size for use as immunoassay reagents or as cell markers or labels for microscopic techniques. Applicants' novel colloidal seeded metal sol process includes: preparing gold nuclei or seed using a strong reducing agent in the presence of a stabilizing agent selected so that neither agent will appreciably affect the subsequent growth of the seed produced; preparing metal sol particles of different sizes using seed prepared in accordance with Applicants' method and hydroxylamine or sodium citrate as the reducing agent; inactivating hydroxylamine and its reaction byproducts when hydroxylamine is the reducing agent: coating the particles with a selected material; and using delipidated milk as a post-coater.
Cennerazzo Michael J.
Le Anh V.
Nemore Robert E.
Schutt Ernest G.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
Ortho Diagnostic Systems Inc.
Ortho Diagnostic Systems Inc.
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