Method of producing a recombinant virus

Details Method of producing a recombinant virus Method of producing a recombinant virus

C - Chemistry, Metallurgy

12

N

C12N 15/66 (2006.01)

Patent

CA 2431349

The present invention relates to methods and kits for modifying viral genomes. The method involves introducing into a host cell containing a helper virus, two or more fragments of a first viral genome, the fragments having ends that are capable of being joined together comprising as little as basepair of overlapping sequence. The helper virus is able to facilitate recombination and reactivation of the DNA fragments into active infectious virions.

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