C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) C07H 21/04 (2006.01) C12N 9/10 (2006.01) C12N 15/39 (2006.01)
Patent
CA 2237862
A method of preparation of lysate having .alpha.2,3-sialyltransferase activity is disclosed. A cell culture having essentially no endogenous expression of eukaryotic .alpha.2,3-sialyltransferase is infected with myxoma virus. The harvested triton lysates include significant quantities of a protein having eukaryotic .alpha.2,3-sialyltransferase activity. Compositions having in vitro .alpha.2,3-sialyltransferase activity and comprising a protein extract of lysates of the aforementioned lysates are also provided for.
La présente invention concerne la préparation de lysats possédant une activité .alpha.2,3-sialyltransférase. Le procédé consiste à infecter du virus myxoma une culture cellulaire ne possédant essentiellement pas d'expression endogène d'.alpha.2,3-sialyltransférase eucariote. Les lysats de triton récoltés comportent des quantités importantes d'une protéine possédant une activité .alpha.2,3-sialyltransférase eucariote. L'invention concerne également des compositions possédant une activité .alpha.2,3-sialyltransférase eucariote et comprenant un extrait protidique de lysat appartenant aux lysats mentionnés précédemment.
Commonwealth Scientific And Industrial Research Organisation
Torys Llp
LandOfFree
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