Method of producing an undifferentiated avian cell culture...

Details Method of producing an undifferentiated avian cell culture... Method of producing an undifferentiated avian cell culture...

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) C12N 5/071 (2010.01) C12N 5/0735 (2010.01)

Patent

CA 2381666

A method of producing undifferentiated avian cells expressing an embryonic stem cell phenotype. The method includes the steps of collecting avian gonadal cells comprising primordial germ cells from an avian embryo after the formation of the primitive streak; depositing the avian gonadal cells in contact with a preconditioned feeder matrix; and growing the avian gonadal cells on the pre-conditioned feeder matrix in the presence of media for a time sufficient to produce an avian cell culture consisting essentially of undifferentiated avian cells expressing an embryonic stem cell phenotype.

L'invention concerne un procédé de production de cellules aviaires indifférenciées, exprimant un phénotype de cellule souche embryonique. Ce procédé comporte les étapes suivantes: recueillement de cellules aviaires gonadiques comprenant des cellules primordiales, à partir d'un embryon aviaire, après formation de la ligne primitive; dépôt des cellules aviaires gonadiques au contact d'une matrice d'alimentation préconditionnée; et culture des cellules aviaires gonadiques sur la matrice d'alimentation préconditionnée, en présence d'un support pendant une période de temps suffisante pour produire une culture de cellule aviaire, essentiellement composée de cellules aviaires indifférenciées, exprimant un phénotype de cellule souche embryonique.

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