Method of producing chondrocytes for transplantation

C - Chemistry – Metallurgy – 12 – N

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C12N 5/077 (2010.01) A61K 35/32 (2006.01) A61L 27/38 (2006.01)

Patent

CA 2522246

It is intended to provide a process by which a large amount of normal human cartilage cells or cell masses can be quickly obtained without any fear of viral or bacterial infection. Namely, a process for producing human cartilage cells characterized by comprising culturing cartilage cells obtained from a cartilage having perichondrium such as an auricular cartilage together with the perichondrium; and a process for producing human cartilage cells characterized by comprising sowing cells to be cultured once or more to give a single-layered or double-layered structure and then culturing the cells to give cartilage cell masses.

Cette invention vise à obtenir un procédé grâce auquel une grande quantité de cellules ou de masses de cellules de cartilage humain normales peuvent être produites rapidement sans risque d'infection virale ou bactérienne. Cette invention concerne plus précisément un procédé servant à produire des cellules de cartilage humain et se caractérisant en ce qu'il consiste à cultiver des cellules de cartilage obtenues à partir d'un cartilage ayant un périchondre tel qu'un cartilage articulaire associé au périchondre ; ainsi qu'à un procédé servant à produire des cellules de cartilage humain et se caractérisant en ce qu'il consiste à ensemencer les cellules à cultiver une ou plusieurs fois, pour former une structure monocouche ou à deux couches, puis à cultiver ces cellules pour produire les masses de cellules de cartilage.

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