Method of producing differentiated progenitor cells by...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/00 (2006.01) C12N 5/071 (2010.01) C12N 5/0735 (2010.01) A61K 35/12 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2388497

An improved method of producing differentiated progenitor cells comprising obtaining inner cell mass cells from a blastocyst and inducing differentiation of the inner cell mass cells to produce differentiated progenitor cells. The differentiated progenitor cells may be transfected such that there is an addition, deletion or alteration of a desired gene. The differentiated progenitor cells are useful in cell therapy and as a I source of cells for the production of tissues and organs for transplantation. Also provided is a method of producing a lineage-defective human embryonic stem cell.

La présente invention concerne un procédé amélioré de production de cellules souches différenciées. Ce procédé consiste à obtenir des cellules de masse cellulaire interne à partir d'un blastocyste et à induire la différenciation des cellules de masse cellulaire interne, afin de produire des cellules souches différenciées. Lesdites cellules souches différenciées peuvent être transfectées de façon à obtenir une addition, une suppression ou une modification du gène souhaité. Les cellules souches différenciées sont utilisées en thérapie cellulaire et en tant que source de cellules dans la production de tissus et d'organes destinés à la transplantation. La présente invention concerne également un procédé de production d'une cellule embryonnaire humaine à lignée défectueuse.

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