Method of producing foreign gene products

Details Method of producing foreign gene products Method of producing foreign gene products

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.2

C12N 15/86 (2006.01) C07K 14/56 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01)

Patent

CA 1309044

ABSTRACT OF THE DISCLOSURE A method of producing foreign gene products which comprises (1) transforming animal cells either (a) by using two plasmids, namely a plasmid containing the MMTV LTR and a foreign gene encoding a physiologically active substance inserted therein downstream from the LTR and a plasmid containing the LTR and a glucocorticoid receptor protein gene inserted therein downstream from the LTR, or (b) by using one plasmid comprising the LTR, a glucocorticoid receptor protein gene located downstream from the LTR, another LTR and a foreign gene encoding a physiologically active substance located downstream from the latter LTR, and (2) cultivating the transformed animal cell-s in a medium containing glucocorticoid in an amount effective for inducing mRNA transcription.

582142

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