Method of producing haploid and doubled haploid plant embryos

Details Method of producing haploid and doubled haploid plant embryos Method of producing haploid and doubled haploid plant embryos

A - Human Necessities

01

H

A01H 1/08 (2006.01) A01H 1/02 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01)

Patent

CA 2611021

The invention relates to a method for producing haploid plant embryos, comprising providing microspores or pollen that comprise cell division inducing molecules; pollinating an embryo sac cell, in particular an egg cell, of the plant of which the haploid embryo is to be made with the microspores or pollen; allowing the microspores or pollen to discharge the cell division inducing molecules in or in the vicinity of the embryo sac cell, in particular the egg cell, to trigger division thereof to obtain a haploid plant embryo. When doubled haploid plant embryos are to be produced doubling of the chromosome number takes place at a certain stage after pollination, in particular during cell division or after obtaining the embryo. The invention further relates to the embryos thus obtained, plants regenerated therefrom and progeny thereof.

La présente invention concerne un procédé de production d'embryons de plantes haploïdes consistant à utiliser des microspores ou du pollen qui comprennent des molécules induisant la division cellulaire; à polliniser une cellule du sac embryonnaire, en particulier une cellule oeuf, de la plante à partir de laquelle l'embryon haploïde va être conçu à l'aide des microspores ou du pollen; et à permettre aux microspores ou au pollen de décharger les molécules induisant la division cellulaire dans la cellule du sac embryonnaire ou à proximité de celle-ci, en particulier la cellule oeuf, pour déclencher la division de celle-ci afin d'obtenir un embryon de plante haploïde. Lorsque des embryons de plantes dihaploïdes doivent être produits, la duplication du nombre de chromosomes a lieu à un certain moment après la pollinisation, en particulier pendant la division cellulaire ou après l'obtention de l'embryon. Cette invention concerne également les embryons ainsi obtenus, les plantes régénérées à partir de ceux-ci, et la descendance de celles-ci.

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