Method of producing heterogeneous proteins

C - Chemistry – Metallurgy – 12 – P

Patent

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Details

C12P 21/00 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2695849

The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses alanine aminotransferase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide.

L'invention concerne un procédé de production à haut rendement d'une protéine naturelle ou recombinée. L'invention concerne plus particulièrement un procédé de production d'un polypeptide, consistant à cultiver des cellules qui expriment fortement une alanine aminotransférase et contiennent un ADN codant le polypeptide transféré souhaité, ce qui leur permet de produire le polypeptide souhaité.

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